11.2022 - 02.2026

Validazione di modelli sperimentali di ischemia cerebrale

In this project, in vitro preclinical models of cerebral ischemia were developed. Among these, the most important are hippocampal organotypic slices, prepared by newborn rats and grown in vitro for two weeks until ripe. In these slices, which maintain the typical anatomical architecture of the brain in vivo, it is possible to simulate ischemia by subjecting it to oxygen and glucose deprivation (OGD) for 30 minutes. 24 hours after this insult, a characteristic lesion can be observed in the CA1 region. Another model is acute hippocampal slices taken from adult rats and incubated for the time necessary to observe and evaluate, after a 30-minute OGD, typical ischemic electrophysiological alterations such as anoxic depolarization.

The possibility of being able to use biochemistry, molecular biology, electrophysiology and confocal and electronic microscopy approaches makes these models very versatile tools for evaluating the morpho-functional alterations that result from a cerebral ischemic insult. For example, these models have been used (see sheet A of the same contact person) to identify and characterize the molecular intracellular pathways compromised during cerebral ischemia and the mechanisms that can lead to neurodegeneration phenomena. Furthermore, in such models it was also possible to evaluate the protective efficacy of some drugs (always see sheet A of the same contact person) that interfere with these mechanisms. 

Settori Scientifico Disciplinari

BIO/14  PHARMACOLOGY

Spoke 6 : Neurodegeneration, Trauma and Stroke